| 30 Oct 26 | r16        | AFC FyldeAFC Fylde | 3 - 1 | HornchurchHornchurch |
| 31 Oct 26 | r16        | Forest Green RoversForest Green | 2 - 2 | Harrogate TownHarrogate Tow |
| 31 Oct 26 | r16        | Halifax TownHalifax Town | 2 - 0 | Yeovil TownYeovil Town |
| 31 Oct 26 | r16        | Aldershot TownAldershot Tow | 1 - 0 | GatesheadGateshead |
| 31 Oct 26 | r16        | BarrowBarrow | 1 - 1 | EastleighEastleigh |
| 31 Oct 26 | r16        | Boreham WoodBoreham Wood | 4 - 3 | Hartlepool UnitedHartlepool Un |
| 31 Oct 26 | r16        | Solihull MoorsSolihull Moor | 2 - 1 | Kidderminster HarriersKidderminster |
| 31 Oct 26 | r16        | AltrinchamAltrincham | 2 - 4 | Carlisle UnitedCarlisle Unit |
| 31 Oct 26 | r16        | TamworthTamworth | 0 - 1 | WokingWoking |
| 31 Oct 26 | r16        | WorthingWorthing | 0 - 3 | Sutton UnitedSutton United |
| 31 Oct 26 | r16        | WealdstoneWealdstone | 3 - 2 | Southend UnitedSouthend Unit |
| 31 Oct 26 | r16        | Boston UnitedBoston United | 2 - 1 | Scunthorpe UnitedScunthorpe Un |