| 12 Sep 26 | r09        | BuryBury | 4 - 0 | EmleyEmley |
| 12 Sep 26 | r09        | GuiseleyGuiseley | 4 - 1 | QuornQuorn |
| 12 Sep 26 | r09        | Gainsborough TrinityGainsborough | 0 - 2 | AvroAvro |
| 12 Sep 26 | r09        | Hyde UnitedHyde United | 1 - 3 | Whitby TownWhitby Town |
| 12 Sep 26 | r09        | WorkingtonWorkington | 3 - 1 | Ilkeston TownIlkeston Town |
| 12 Sep 26 | r09        | Ashton UnitedAshton United | 0 - 1 | Cleethorpes TownCleethorpes T |
| 12 Sep 26 | r09        | Bamber BridgeBamber Bridge | 1 - 0 | Alfreton TownAlfreton Town |
| 12 Sep 26 | r09        | Lancaster CityLancaster Cit | 0 - 2 | Curzon AshtonCurzon Ashton |
| 12 Sep 26 | r09        | FC United of ManchesterFC United of | 4 - 1 | Warrington TownWarrington To |
| 12 Sep 26 | r09        | Warrington RylandsWarrington Ry | 1 - 1 | Stockton TownStockton Town |
| 12 Sep 26 | r09        | Redcar AthleticRedcar Athlet | 3 - 2 | Leek TownLeek Town |