| 06 Oct 26 | r12        | Alfreton TownAlfreton Town | 1 - 0 | FC United of ManchesterFC United of |
| 06 Oct 26 | r12        | Curzon AshtonCurzon Ashton | 3 - 1 | Redcar AthleticRedcar Athlet |
| 06 Oct 26 | r12        | Ilkeston TownIlkeston Town | 0 - 2 | GuiseleyGuiseley |
| 06 Oct 26 | r12        | Leek TownLeek Town | 3 - 3 | Warrington RylandsWarrington Ry |
| 06 Oct 26 | r12        | Cleethorpes TownCleethorpes T | 1 - 0 | Hyde UnitedHyde United |
| 06 Oct 26 | r12        | Warrington TownWarrington To | 2 - 2 | WorkingtonWorkington |
| 06 Oct 26 | r12        | Whitby TownWhitby Town | 2 - 6 | Gainsborough TrinityGainsborough |
| 06 Oct 26 | r12        | QuornQuorn | 2 - 2 | Ashton UnitedAshton United |
| 06 Oct 26 | r12        | Stockton TownStockton Town | 1 - 0 | BuryBury |
| 07 Oct 26 | r12        | AvroAvro | 5 - 3 | Lancaster CityLancaster Cit |
| 03 Nov 26 | r12        | EmleyEmley | 2 - 0 | Bamber BridgeBamber Bridge |