| 10 Oct 26 | r13        | Alfreton TownAlfreton Town | 2 - 3 | BuryBury |
| 10 Oct 26 | r13        | Curzon AshtonCurzon Ashton | 2 - 0 | Hyde UnitedHyde United |
| 10 Oct 26 | r13        | Ilkeston TownIlkeston Town | 0 - 5 | Redcar AthleticRedcar Athlet |
| 10 Oct 26 | r13        | Leek TownLeek Town | 2 - 2 | Ashton UnitedAshton United |
| 10 Oct 26 | r13        | Cleethorpes TownCleethorpes T | 3 - 0 | Warrington RylandsWarrington Ry |
| 10 Oct 26 | r13        | Warrington TownWarrington To | 2 - 2 | Lancaster CityLancaster Cit |
| 10 Oct 26 | r13        | Whitby TownWhitby Town | 0 - 0 | Bamber BridgeBamber Bridge |
| 10 Oct 26 | r13        | AvroAvro | 0 - 1 | GuiseleyGuiseley |
| 10 Oct 26 | r13        | QuornQuorn | 3 - 0 | WorkingtonWorkington |
| 10 Oct 26 | r13        | Stockton TownStockton Town | 2 - 2 | FC United of ManchesterFC United of |
| 10 Oct 26 | r13        | EmleyEmley | 1 - 2 | Gainsborough TrinityGainsborough |