| 31 Oct 26 | r16        | Havant and WaterloovilleHavant and Wa | 1 - 0 | Taunton TownTaunton Town |
| 31 Oct 26 | r16        | Frome TownFrome Town | 2 - 3 | Chichester CityChichester Ci |
| 31 Oct 26 | r16        | UxbridgeUxbridge | 2 - 0 | Yate TownYate Town |
| 31 Oct 26 | r16        | Chertsey TownChertsey Town | 4 - 3 | Basingstoke TownBasingstoke T |
| 31 Oct 26 | r16        | Evesham UnitedEvesham Unite | 3 - 2 | Bracknell TownBracknell Tow |
| 31 Oct 26 | r16        | SholingSholing | 4 - 2 | Malvern TownMalvern Town |
| 31 Oct 26 | r16        | Gosport BoroughGosport Borou | 3 - 2 | Hanwell TownHanwell Town |
| 31 Oct 26 | r16        | Wimborne TownWimborne Town | 2 - 0 | Chippenham TownChippenham To |
| 31 Oct 26 | r16        | Gloucester CityGloucester Ci | 2 - 2 | BerkhamstedBerkhamsted |
| 31 Oct 26 | r16        | Bath CityBath City | 1 - 0 | Poole TownPoole Town |
| 31 Oct 26 | r16        | Hanworth VillaHanworth Vill | 4 - 2 | Plymouth ParkwayPlymouth Park |